Applications of CRISPR/Cas9 for Biotic and Abiotic Stress Resistance of Rice (Oryza sativa)

Biotechnology has played an important role in the development of varieties with enhanced biotic and abiotic stress tolerance, higher yield potential and an improved resistance towards major diseases and pathogens. The precision of the CRISPR-Cas9 system's target recognition is notably dependable because it employs the Watson and Crick model to identify target sites and utilizes sequence analysis to pinpoint potential off-target locations. In a study carried out to develop resistance against bacterial blight on CO51 variety of rice, a single guide RNA was designed using the structure and sequence information obtained through Ensembl Plants database. The integration of CRISPR/Cas9 technology with traditional breeding methods and other advanced biotechnological tools may further enhance the development of stress-resilient rice varieties with improved productivity and sustainability.